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Vol 56(2022) N 6 p. 1012-1019; DOI 10.1134/S0026893322060206  R. Wang1,2, M.-Y. Shi1, B.-B. Ma1,2, J. Sheng1,2* Mechanism of Leakage in Phosphatidylserine-Containing Membranes by Melittin 1Xinjiang Laboratory of Phase Transitions and Microstructures of Condensed Matter Physics, College of Physical Science and Technology, Yili Normal University, Yining, 835000 China2Key Laboratory of Micro-nano Electric Sensing Technology and Bionic Devices, Yili Normal University, Yining, 835000 China *2235835064@qq.com Received - 2022-04-06; Revised - 2022-04-25; Accepted - 2022-05-03 Phosphatidylserine (PS) is an important apoptotic-cell surface signal that exists in bacterial and cancer cells. The mechanism by which melittin interacts with the PS membrane remains unclear. Here, we revealed this mechanism by using a dual-channel fluorescence microscope to observe the concentration-dependent process of pore formation in giant unilamellar vesicles (GUVs) that were exposed to melittin solution. We found that unsaturated PS membranes differed significantly from saturated PS membranes in different phases. This study provides a reference for research and development of anticancer drugs. melittin, phosphatidylserine lipid bilayer, peptide-induced permeation, concentration dependence, giant unilamellar vesicle |
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