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Vol 56(2022) N 6 p. 950-962; DOI 10.1134/S0026893322060218  A.A. Zagoskin1, M.V. Zakharova1, M.O. Nagornykh1,2* Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery 1Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142290 Russia2Sirius University of Science and Technology, Sirius, Sochi, 354349 Russia *derbanner@gmail.com Received - 2022-05-18; Revised - 2022-06-25; Accepted - 2022-06-28 Gene editing with programmable nucleases opens new perspectives in important practice areas, such as healthcare and agriculture. The most challenging problem for the safe and effective therapeutic use of gene editing technologies is the proper delivery and expression of gene editors in cells and tissues of different organisms. Virus-based and nonviral systems can be used for the successful delivery of gene editors. Here we have reviewed structural elements of nonviral DNA- and RNA-based expression vectors for gene editing and delivery methods in vitro and in vivo. expression DNA vector, IVT RNA, mRNA-based delivery, genome editing, Cas9, ZFN, TALEN, gene therapy, nanoparticles |
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