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Vol 53(2019) N 2 p. 256-266; DOI 10.1134/S0026893319020109  Z. Li1,2*, X.M. Liu2, A.Y. Li2, X.X. Du2, X.B. Wang2, J.X. Liu2, Z.G. Wang2, Q.Q. Zhang2, H.Y. Yu2** Teleost Type 2 Interleukin-1 Receptor (IL-1R2) from the Spotted Halibut (Verasper variegatus): 3D Structure and a Role in Immune Response 1School of Agriculture, Ludong University, Yantai, 264002 P. R. China2Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Science, Ocean University of China, Qingdao, 266003 P. R. China *lizanlxm@163.com **yuhaiyang@ouc.edu.cn Received - 2018-01-27; Revised - 2018-06-07; Accepted - 2018-07-13 The type 2 interleukin-1 receptor (IL-1R2) is one of natural IL-1β singling inhibitors in mammals. We cloned and sequenced the IL-1R2 gene in V. variegatus (VvIL-1R2). The phylogenetic analysis showed that the molecular structure VvIL-1R2 is similar to that of its orthologues in other vertebrates. The expression levels of VvIL-1R2 are relatively high in the peripheral blood leukocytes (PBLs), gill, and spleen. In addition, peculiar expression patterns for his molecule were detected at various developmental stages, implying that in flatfishes the IL-1R2 may have be important for embryonic development and metamorphosis. In PBLs, the treatment with pathogen-associated molecular patterns (PAMPs) induced a significant and rapid up-regulation of VvIL-1R2, pointing at its involvement in the immune responses against bacterial and viral pathogens. IL-1R2, Verasper variegatus, metamorphosis, 3D modeling, PAMPs |
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