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Vol 43(2009) N 1 p. 76-81; Н.Т. Wu1,2, X.М. Yan3, Y.L. Hu1, Z.Y. Diao1, J. Wu3 S.Q. Zhang1 Expression, characterization of recombinant human soluble BAFF secreted from CHO cell 1Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing, 210046 China2Basic Medical College of Nanjing University of Chinese Medicine, Nanjing, 210029 China 3School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China Received - 2007-11-06; Accepted - 2008-02-21 B cell-activating factor of the TNF family (BAFF) is critical for B cell maturation and survival. Here, we constructed a stable CHO cell line, in which the expression level of soluble form of BAFF (sBAFF) was raised from 0.13 μg/ml to 0.55 μg/ml. Purified recombinant sBAFF from these CHO cells not only bound to its receptors but also co-stimulated the proliferation of human peripheral blood B lymphocyte in vitro. These results provided us with a useful basis for further studies about sBAFF-related research. B cell-activating factor of the TNF family, B lymphocyte, Chinese Hamster Ovary cell |
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