Journal of Molecular Biology

Year	IMPACT-FACTOR
2023	1,500
2022	1,200
2021	1,540
2020	1,374
2019	1,023
2018	0,932
2017	0,977
2016	0,799
2015	0,662
2014	0,740
2013	0,739
2012	0,637
2011	0,658
2010	0,654
2009	0,570
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2007	0,805
2006	0,330
2005	0,435
2004	0,623
2003	0,567
2002	0,641
2001	0,490
2000	0,477
1999	0,762
1998	0,785
1997	0,507
1996	0,518
1995	0,502

Vol 45(2011) N 6 p. 950-958;

P.V. Spirin¹, N.A. Nikitenko¹, T.D. Lebedev¹, P.M. Rubtsov¹, C. Stocking², V.S. Prasolov^1*

Modulation of Activated Oncogene c-kit Expression with RNA-Interference
¹Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
²Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg, 20251, Germany

^*prasolov@eimb.ru
Received - 2011-03-30; Accepted - 2011-06-22

Overexpression of oncogene c-kit is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene c-kit was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene c-kit. Further, small hairpin RNAs (shRNAs) targeting c-kit mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in c-kit expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML.

RNA-interference, small interfering RNA (siRNA), small hairpin RNA (shRNA), lentiviral vectors, regulation of gene expression, posttranscriptional regulation of expression, acute myeloid leukemia (AML)