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Vol 45(2011) N 5 p. 766-773; O.E. Voronko1, E.V. Dmitrieva-Zdorova1*, E.A. Latysheva2, M.G. Aksenova3, G.I. Storozhakov4, N.V. Bodoev1, A.I. Archakov1 CARD15 and TLR4 Polymorphisms in Atopic Bronchial Asthma 1Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia2Institute of Immunology, Federal Medical Biological Agency of Russia, Moscow, 117997, Russia 3Sysin Institute for Human Ecology and Environmental Health, Russian Academy of Medical Sciences, Moscow, 119992, Russia 4Pirogov Russian State Medical University, Moscow, 117997, Russia *inst@ibmc.msk.ru Received - 2010-06-28; Accepted - 2011-01-17 In order to investigate whether single nucleotide polymorphisms G(+2722)C and 3020insC of CARD15 and Asp299Gly of TLR4 contribute to atopic bronchial asthma, we performed a comparative analysis of allele and genotype frequencies of these polymorphisms in Russian patients from Moscow. DNA specimens obtained from 283 patients with atopic bronchial asthma and 227 healthy donors were genotyped. Neither G(+2722)C or 3020insC in CARD15 nor Asp299Gly in TLR4 were associated with asthma; CARD15 polymorphisms were not also associated with asthma severity. A haplotype frequency analysis of CARD15 polymorphisms did not detect significant differences between the groups studied. However, a strong association was found between Asp299Gly and asthma course: the Asp allele was associated with mild disease, while the minor Gly allele was associated with moderate/severe asthma (OR = 0.47, 95% CI [0.24-0.93] and OR = 2.12, 95% CI [1.08-4.18], respectively). atopic bronchial asthma, MALDI-TOF mass spectrometry, CARD15 polymorphisms G(+2722)C and 3020insC, TLR4 polymorphism Asp299Gly |
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