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Vol 45(2011) N 4 p. 680-685; E.S. Grigor'eva1*, Y.A. Bukurova2, G.S. Krasnov2, S.G. Afanasev1,3, N.V. Cherdyntseva1, S.A. Tuzikov1, E.L. Choinzonov1,3, V.L. Karpov2, N.A. Lisitsy2, S.F. Beresten2 Identification of Proteins Overexpressed in Malignant Gastric Tumors: Comparison of Results Obtained by 2DE and Bioinformatic Search 1Cancer Research Institute, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634009 Russia2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia 3Siberian State Medical University, Tomsk, 634050 Russia *grigorieva@oncology.tomsk.ru Received - 2010-08-23; Accepted - 2010-11-10 A comparison of protein expression profiles in intestinal and diffuse gastric tumors and normal tissues by 2D gel electrophoresis identified three proteins (SOD2, S100A6, and TXN) overexpressed in tumors. SOD2 and TXN were more frequently overexpressed in diffuse tumors than in intestinal ones. Based on the published data on comparative 2DE protein expression analysis, a control panel of eleven proteins overexpressed in gastric tumors was compiled. A bioinformatic search for the respective mRNAs in the Oncomine database (representing mRNA transcription levels in tumor vs. normal tissues) showed the agreement of proteomic and transcriptomic data for seven of 11 proteins. gastric cancer, proteomics, bioinformatics, proteomic markers of tumors |
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