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Vol 45(2011) N 4 p. 608-613; E.S. Ioudinkova1*, D.A. Bunina2, S.V. Ulyanov2, A.A. Gavrilov1, S.V. Razin1,2 Patterns of Histone Modifications across the Chicken Alfa-Globin Genes Domain 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia2Biological Faculty, Moscow State University, Moscow, 119992 Russia *ioudinkova@inbox.ru Received - 2010-11-12; Accepted - 2010-12-17 Using native chromatin imunoprecipitation (N-ChIP) followed by TaqMan RT-PCR quantitative analysis, we have determined the profiles of histone acetylation and histone methylation within the α-globin gene domain before and after switching of embryonic globin gene expression. The results obtained do not support a supposition that the inactivation of the embryonic-type globin gene in the erythroid cells of the adult lineage is mediated via formation of an inactive chromatin domain. On the other hand, we have demonstrated that suppression of the gene activity in erythroid cells of adult lineage correlates with decrease of the histone acetylation level within the embryonic subdomain of the α-globin gene domain. α-globin genes domain, chromatin, acetylation |
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