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Vol 47(2013) N 6 p. 871-878; Y.V. Gritsyna1, N.N. Salmov1, I.M. Vikhlyantsev1*, A.D. Okuneva 2, M.G. Sharapo3, V.V. Teplova1, Z.A. Podlubnaya1 Changes in Gene Expression and Titin (Connectin) Content in Striated Muscles of Chronically Alcoholized Rats 1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290 Russia2Pushchino State Institute of Natural Sciences , Pushchino, 142290 Russia 3Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, 142290 Russia *vikhlyantsev@iteb.ru Received - 2013-04-17; Accepted - 2013-05-25 Using the real-time reverse-transcription polymerase chain reaction (RT-PCR) and low percentage SDS-gel electrophoresis, changes in the gene expression and isoform composition of a giant sarcomeric protein titin (connectin) in cardiac muscle and changes in its isoform composition in skeletal muscle m. soleus of chronically alcoholized rats were studied. The decreased content of titin protein in examined muscles and decreases in titin gene expression in the myocardium of chronically alcoholized rats were found, which indicates the development of the pathological process. titin isoforms, ttn gene, myosin heavy chains, striated muscles, chronically alcoholized rats |
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