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Vol 51(2017) N 1 p. 108-111; DOI 10.1134/S0026893317010034  A.V. Arkhipov1, A.G. Solovyev1,2, V.K. Vishnichenko1* Persistent Shallot virus X infection correlates with transcriptional repression of plant cell RNA-dependent RNA polymerase and DCL proteins in plant roots 1Institute of Agricultural Biotechnology of the Russian Academy of Sciences, Moscow, 127550 Russia2Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow, 119992 Russia *vish@iab.ac.ru Received - 2016-02-16; Accepted - 2016-02-24 Shallot virus X is a typical representative of Allexiviruses. The transcription levels of principal genes involved in the RNA silencing in healthy and shallot virus X-infected plants have been quantified by real-time polymerase chain reaction. There is a negative correlation between the reproduction rates of RNA virus and the levels of RNA-dependent RNA polymerase and DCL proteins in roots and leaves of infected plants. These observations indicate that Shallot X virus employs noncanonical ways of overcoming the antiviral defense of the plant by systemic RNA silencing. shallot, ShVX, RNA silencing, persistent infection, plant cell RNA-dependent RNA polymerase, DCL proteins, nonspecific lipid transfer peptides (nsLTPs), real-time PCR |
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