The F178 strain of Penicillium canescens is a natural producer of beta-galactosidase and endo-1,4-beta-xylanase. Transcription of the genes bgaS and xylA encoding these proteins is affected by the carbon catabolite repression, which occurs in the filamentous fungi, primarily by the transcriptional repressor CreA. The creA gene of P. canescens was cloned and it was demonstrated that its own transcription is influenced by the carbon catabolic repression. Interestingly, the CreA protein remains to be nuclear localized irrespectively of the nature of the carbon source and glucose concentration in the medium. In vitro experiments revealed four CreA binding sites localized within promoter of the bgaS gene, four sites in the xylA gene promoter, and one site in the creA gene promoter.