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Vol 50(2016) N 6 p. 880-886; DOI 10.1134/S0026893316050022  O.Y. Agapova1, Y.S. Skoblov2*, G.A. Tkachev3, N.A. Mironova3, S.P. Golitsyn3, V.P. Masenko3, I.E. Chazova3, K.A. Zykov1 Changes in the receptor activity of β2-adrenoreceptors of human T-lymphocytes under the effect of β2-agonists 1Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, 127473 Russia2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia 3Russian Cardiology Research and Production Complex, Moscow, 121552 Russia *sur@ibch.ru Received - 2015-10-21; Accepted - 2015-12-17 Changes in the activity of β2-adrenergic receptors of human T-lymphocytes under the effect of salbutamol (a short-acting β2-agonist) have been evaluated with a new modified radioligand method utilizing [125I]cyanopindolol and a specific ligand ICI 118551. In healthy volunteers, the receptor activity decreased after 30 min upon the inhalation of salbutamol and restored to the initial level after 2 h. At the same time, there were changes in the transcription level of the ADRB2 gene, which encodes the protein component of the β2-adrenoreceptor. The dynamics of β2-adrenergic receptor activity of T-lymphocytes after salbutamol treatment in patients with cardiorespiratory pathology significantly differed from that in healthy volunteers. radioligand binding assay, activity of β2-adrenoreceptors, ADRB2, gene expression, T-lymphocytes |
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