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Vol 44(2010) N 4 p. 536-540; R.N. Ma1, C.J. Deng1, X.M. Zhang1, X.P. Yue1, X.Y. Lan1, H. Chen1,2, C.Z. Lei1* A Novel SNP of α-Lactalbumin Gene in Chinese Dairy Goats 1Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University Yangling, Shaanxi, 712100, China2nstitute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, China *leichuzhao1118@126.com Received - 2009-10-26; Accepted - 2009-12-17 The α-lactalbumin (α-LA) plays a key role in lactose synthesis in mammary glands of domestic animals. Mutations in the α-LA gene are associated with the milk traits in dairy cattle. In our study, a novel SNP: NO_X06366: g.875 C > T was detected in 708 dairy goat individuals - 268 of the Xinong Saanen breed and 440 of Guanzhong breed, which revealed a synonymous mutation in the exon 1 of α-LA gene. The Polymerase Chain Reaction--Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing techniques showed that there were three genotypes: CC, CT and TT. Moreover, the χ2-test showed that the genotype frequencies of the two breeds were in good agreement with the Hardy-Weinberg equilibrium (P > 0.05). The relationship of the polymorphism of dairy goat α-LA gene with the milk trait and the body size trait was revealed. Individuals with the CC genotype were significantly smaller at chest circumference than those with CT (P < 0.05) in both breeds. But the milk trait and other body size traits of the two dairy goat breeds had no significant association with genotypes studied. dairy goat, α-lactalbumin gene, PCR-SSCP, SNP, body size traits, milk trait |
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