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Vol 50(2016) N 4 p. 615-620; DOI 10.1134/S0026893316040130  N.V. Soshnikova1, N.E. Vorob'eva1, A.A. Kolacheva2*, D.Y. Gurskiy1, R.R. Nigmatullina4,5, Z.A. Zalyalova4,6, S.G. Georgieva1,3, M.V. Ugrumov2 Ratio of transcription factor PHF10 splice variants in lymphocytes as a molecular marker of Parkinson's disease 1Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia2Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334 Russia 3Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia 4Kazan State Medical University, Ministry of Health of the Russian Federation, Kazan, 420012 Russia 5Сenter of Early Diagnosis of Neurodegenerative Disorders, Kazan, 420100 Russia 6Center for Extrapyramidal Pathology and Botulinum Therapy, Kazan Hospital for War Veterans, Ministry of Health of the Republic of Tatarstan, Kazan, 420039 Russia *annakolacheva@gmail.com Received - 2016-02-11; Accepted - 2016-02-11 Parkinson's disease (PD) is the second most common neurodegenerative disorder and causes degeneration of dopaminergic neurons in the nigrostriatal system of the brain. PHF10 is one of the most important regulatory subunits of the SWI/SNF chromatin-remodeling protein complex, which controls the gene function and chromatin state in neurons. Two alternative RHF10 isoforms, PHF10-P and PHF10-S, replace each other in the complex to change the target gene pattern. Expression of the PHF10-P and PHF10-S transcripts in the nigrostriatal system and their ratio in blood lymphocytes were found to change in a mouse model of early clinical stage of PD as compared with control mice. Changes in PHF10-S level were also observed in peripheral blood lymphocytes from patients with early clinical stage of PD. A ratio of the PHF10-P and PHF10-S transcripts in peripheral blood cells was assumed to provide a potential marker of early stage PD. Parkinson's disease, RHF10 isoforms, Parkinson's disease peripheral marker |
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