Journal of Molecular Biology

Year	IMPACT-FACTOR
2023	1,500
2022	1,200
2021	1,540
2020	1,374
2019	1,023
2018	0,932
2017	0,977
2016	0,799
2015	0,662
2014	0,740
2013	0,739
2012	0,637
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2010	0,654
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2007	0,805
2006	0,330
2005	0,435
2004	0,623
2003	0,567
2002	0,641
2001	0,490
2000	0,477
1999	0,762
1998	0,785
1997	0,507
1996	0,518
1995	0,502

Vol 42(2008) N 3 p. 381-390;

Keji Jiang¹, Zhihua Liao², Yan Pi¹, Zhuoshi Huang¹, Rong Hou¹, Ying Cao¹, Qing Wang¹, Xiaofen Sun¹, Kexuan Tang^1,3

Molecular cloning and expression profile of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Hyoscyamus niger
¹State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R & D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China
²Laboratory of Natural Products and Metabolic Engineering, Chongqing Sweet Potato Research Center, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, People's Republic of China
³Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R & D Center, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China
Received - 2007-05-18; Accepted - 2007-06-16

Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named tropane alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary metabolites. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession no.: AY708383), which was 1044 bp long, with a 747-bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues. Southern blot analysis indicated that it was a multicopy gene. RT-PCR analysis revealed that the expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis of jasmonates.

allene oxide cyclase, Hyoscyamus niger, jasmonate, RT-PCR, secondary metabolite