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Vol 47(2013) N 3 p. 352-357; M.S. Nazarenko1,2*, A.V. Markov1, I.N. Lebedev1,2, A.A. Sleptsov1, A.V. Frolov3, O.L. Barbarash3, L.S. Barbarash3, V.P. Puzyrev1,2 DNA Methylation Profiling of the Vascular Tissues in the Setting of Atherosclerosis 1Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634050 Russia2Siberian State Medical University, Ministry of Healthcare of the Russian Federation, Tomsk, 634050 Russia 3Research Institute for Complex Studying of Cardiovascular Diseases, Russian Academy of Medical Sciences, Kemerovo, 650002 Russia *maria.nazarenko@medgenetics.ru Received - 2012-09-26; Accepted - 2012-10-11 Currently, the question of epigenetic mechanisms of gene regulation in the context of cardiovascular diseases is of considerable interest. Here, DNA methylation profiles of vascular tissues of atherosclerotic patients have been analyzed for the first time using the Infinium Human Methylation27 BeadChip microarray (Illumina, United States). As the result, within 286 genes, 314 CpG sites that varied significantly in the level of DNA methylation between the tissue samples of carotid (in the area of atherosclerotic plaques and nearby macroscopically intact tissues of the vascular wall) and mammary arteries, as well as saphenous veins have been identified. The most pronounced differences in the methylation level was registered for CpG sites of homeobox genes HOXA2 and HOXD4, as well as the imprinted MEST gene. In particular, these genes were found to be hypomethylated in the carotid atherosclerotic plaques compared to their methylation patterns in intact tissues of internal mammary arteries and saphenous veins. DNA methylation, atherosclerosis, Infinium Human Methylation27 BeadChip |
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