In this study, we evaluated the level of c-kit, VEGFA, and MYC gene expression in seven neuroblastoma stable cell lines, i.e., SK-N-SH, SK-N-BE, SK-N-AS, SH-SY5Y, Kelly, IMR-32, and LAN-1. The level of expression of these genes can serve as a diagnostic factor for cancer progression and proteins encoded by these genes are promising targets for neuroblastoma treatment. SH-SY5Y and SK-N-AS cells have the highest MYC expression and the same VEGFA expression, although SH-SY5Y has tenfold higher c-kit expression than SK-N-AS cells. Both IMR-32 and LAN-1 cells have low levels of MYC expression, but differ in c-kit expression, while IMR-32 has significantly higher c-kit expression than any other neuroblastoma cell line. On the other hand, LAN-1 has the highest VEGFA expression. These data suggest that MYC, c-kit, and VEGFA genes can play different roles in the development and progression of neuroblastoma depending on other activated molecular mechanisms in malignant cells.