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Vol 47(2013) N 2 p. 222-227; Panpan Ye, Kana Lin, Zhaochun Li, Jian Liu, Ke Yao, Wen Xu* (-)-Epigallocatechin Gallate Regulates Expression of Apoptotic Genes and Protects Cultured Human Lens Epithelial Cells under Hyperglycemia Eye Center of Second Affiliated Hospital, College of Medicine, Zhejiang University, Hang Zhou, 310009, China*xuwenhz2002@yahoo.com.cn Received - 2012-03-29; Accepted - 2012-07-24 (-)-Epigallocatechin gallate (EGCG), the most abundant component in green tea, has a potent anti-apoptotic activity. The purpose of this study was to investigate the protective effects of EGCG and their molecular mechanisms on high glucose-induced apoptosis of human lens epithelial cells (HLEB-3). HLEB-3 cells were exposed to various concentrations of glucose and EGCG. Cell death was assessed by MTT assay and flow cytometry using annexin V and propidium iodide. The expression of the Bcl-2 family, c-fos, c-myc and p53 was measured by real-time PCR. EGCG decreased the Bcl-2/Bax expression stimulated by a high glucose. Moreover, EGCG suppressed the high glucose-induced expression of c-fos, c-myc and p53. These findings suggest that EGCG protects HLEB-3 cells from high glucose-induced apoptosis by regulating the gene expression of the Bcl-2 family, c-fos, c-myc and p53. Thus, EGCG may have a potential protective effect against diabetic cataract formation. apoptosis, (-)-epigallocatechin gallate, glucose, human lens epithelial cells |
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