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Vol 48(2014) N 4 p. 607-612; DOI 10.1134/S0026893314040098  I.A. Popov1,2,3,4,5, M.I. Indeikina1,2,3, S.I. Pekov1,3, N.L. Starodubtseva1,3,4, A.S. Kononikhin1,3,4, M.I. Nikolaeva1, E.N. Kukaev1,3,4, Y.I. Kostyukevich1,4, S.A. Kozin2,5, A.A. Makarov2, E.N. Nikolaev1,3,4,5* Estimation of Phosphorylation Level of Amyloid-Beta Isolated from Human Blood Plasma: Ultrahigh-Resolution Mass Spectrometry 1Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia 3Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia 4Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow Region, 141700 Russia 5Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121 Russia *nikolaev@chph.ras.ru Received - 2014-03-07; Accepted - 2014-03-13 Recently, amyloid-beta (Aβ) phosphorylation at position 8 has been shown to be associated with pathogenesis of Alzheimer's disease. Since the modification occurs in the key fragment of the metal-binding domain of Aβ and should seriously affect the interaction of pS8-Aβ with zinc ions, this isoform may be a potential precursor of pathogenic oligomer forms of Aβ. Hence the level of pS8-Aβ in human biological fluids (blood, urine, cerebrospinal fluid) may reflect various stages of pathogenesis of the Alzheimer's disease. The aim of the work was to develop a prototype of an analytical method for quantitative determination of the level of pS8-Aβ isoform in binary mixtures with native Aβ in order to further use it to estimate the levels of phosphorylated amyloid-beta in blood plasma samples of patients diagnosed with Alzheimer's disease. phosphorylation, amyloid-beta, mass spectrometry, quantitative evaluation |
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