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Vol 46(2012) N 3 p. 457-466; O.A. Stepanov1, M.M. Prokofeva1, C. Stocking2, V.P. Varlamov3, A.N. Levov3, G.A. Vikhoreva4, P.V. Spirin1, S.N. Mikhailov1*, V.S. Prasolov1** Replication-Competent Gamma-Retrovirus Mo-MuLV Expressing Green Fluorescent Protein as Efficient Tool for Screening of Inhibitors of Retroviruses That Use Heparan Sulfate as Primary Cell Receptor 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 1199912Heinrich Pette Institute - Leibniz Institute for Experimental Virology, Hamburg, 20251, Germany 3Center Bioengineering, Russian Academy of Sciences, Moscow, 117312 4Kosygin Moscow State Textile University, Moscow, 119071 *smikh@eimb.ru **prasolov@eimb.ru Received - 2011-09-16; Accepted - 2011-11-24 The effect of sulfated polysaccharides on the efficiency of the infection of mouse transplantable fibroblast SC-1 and NIH-3T3 cell lines by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV), which carries the eGFP gene, was investigated. It was found that sulfated polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH-3T3 cells in the concentration range of 0.01-100 чg/mL and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit Mo-MuLV infection that prevents the further development of viral infection. It was shown that sulfated polysaccharides are also effective inhibitors of other retroviruses, including lentiviruses, which use sulfated polysaccharides as primary cell receptors. Moloney murine leukemia virus (Mo-MuLV), sulfated polysaccharides, chitosan, antiviral activity, heparan sulfate, mouse fibroblast cell lines SC-1 and NIH-3T3 |
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