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Vol 48(2014) N 4 p. 563-572; DOI 10.1134/S002689331404013X  O.S. Shubernetskaya1, D.A. Skvortsov1*, S.A. Evfratov1, M.P. Rubtsova1, E.V. Belova1, O.S. Strelkova 2, V.D. Cherepanynets2, O.A. Zhironkina2, A.M. Olovnikov4, M.E. Zvereva1, I.I.Kireev2, O.A.Dontsova1, 3 High Expression Levels and Nuclear Localization of Novel Danio rerio ncRNA Transcribed from a Genomic Region Containing Repetitive Elements 1Department of Chemistry, Moscow State University, Moscow, 119991 Russia;2Department of Biology, Moscow State University, Moscow, 119991 Russia 3Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia 4Belozerskii Institute of Physicochemical Biology, Moscow State University, Moscow, 119992 Russia *skvorratd@mail.ru Received - 2014-01-31; Accepted - 2014-02-04 Noncoding and repetitive sequences make up a large part of the genome of high eukaryotes, but the elucidation of their roles and mechanisms of action are poorly understood. In this work, we found that interstitial telomeric repeats in the genome of Danio rerio colocalize with repetitive elements, including hAT and EnSpm, which are widely represented in vertebrate genomes. The investigation of a genomic region containing two pairs of these repeats located in close proximity showed that the area is transcribed. RNA-dependent structures containing this sequence were identified in D. rerio fibroblast nuclei, which indicates the functional importance of genomic repetitive elements or their transcripts. repetitive elements, noncoding RNA, chromatin organization, interstitial telomeric repeats, fluorescence in situ hybridization, ageing, fibroblasts, Danio rerio |
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