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Vol 57(2023) N 1 p. 101-104; DOI 10.1134/S0026893323010053  A.A. Kudryavtseva1*, V.A. Alekhin1, M.D. Lebedeva2, E. Cséfalvay3, M. Weiserova4, I.V. Manukhov1,5 Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease 1Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow oblast, 141707 Russia2Autonomous non-profit educational organization "Phystech Lyceum" named after P.L. Kapitsa, Dolgoprudny, Moscow oblast, 141707 Russia 3Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences of the Czech Republic, Zamek 136, Nove Hrady, 37333 Czech Republic 4Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Praha 4, 14220 Czech Republic 5Moscow State University of Food Production, Moscow, 125080 Russia *kudryavtseva@phystech.edu Received - 2022-07-30; Revised - 2022-08-08; Accepted - 2022-08-08 ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown; the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems. antirestriction, RM-I, ArdB, EcoAI |
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