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Vol 53(2019) N 6 p. 862-875; DOI 10.1134/S0026893319060116  N.A. Lomov1,2*, V.S. Viushkov1,2, A.P. Petrenko1, M.S. Syrkina1,2, M.A. Rubtsov1,2,3** Methods of Evaluating the Efficiency of CRISPR/Cas Genome Editing 1Moscow State University, Moscow, 119991 Russia2LIA LFR20 Laboratoire Franco-Russe de Recherches en Oncologie, Moscow State University, Moscow, 119991 Russia 3Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119991 Russia *lomov13@gmail.com **ma_rubtsov@mail.ru Received - 2019-05-16; Revised - 2019-06-03; Accepted - 2019-07-04 The CRISPR/Cas system is currently widely used for genome editing. The procedure of genome editing includes two necessary steps: (i) searching for the most effective guide RNA, and (ii) analyzing clones for presence of the desired mutation. This review presents the methods used to assess the efficiency of the CRISPR/Cas system and to confirm mutation in the target locus and discusses their advantages and disadvantages. It aims to provide information that could help researchers to choose a technique most appropriate for their specific tasks and available resources. CRISPR/Сas9, NGS, droplet digital PCR, RFLP, T7E1, SURVEYOR, TIDE, IDAA, pDR/FACS, ENIT, SNP, HRMA, SSCP, cbPCR, DHPLC, LDR |
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