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Vol 46(2012) N 2 p. 328-331; D.V. Vinogradov1*, O.V. Tsoy1,2, A.V. Zaika1,2, A.V. Lobanov3, A.A. Turanov3, V.N. Gladyshev3, M.S. Gelfand1,2 Draft Macronucleus Genome of Euplotes crassus Ciliate 1Kharkevich Institute of Information Transmission Problems, Russian Academy of Sciences, Moscow, 127994 Russia2Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119991 Russia 3Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA *dimavin@bioinf.fbb.msu.ru Received - 2011-06-06; Accepted - 2011-09-28 Basic bioinformatics analysis has been performed on the draft macronucleus genome and transcriptome of the Euplotes crassus ciliate. More than a quarter of E. crassus genes contain several exons. Among introns, a large fraction of tiny introns was revealed with the length of 20-30 nucleotides. We determined 63 cases of alternative splicing and found 14 introns with nonstandard splicing sites. About 2000 of hypothesized genes in E. crassus have no homologues with other ciliates but in the majority of them a considerable similarity is observed with bacterial genes that can be due to contamination while ciliate sample preparation. Comparison of the genomes of E. crassus and other ciliates evidences the expansion of the same gene families, which are responsible for the vitality of free-living heterotrophic organisms. genome analysis, ciliate, genome, bioinformatics |
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