2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 52(2018) N 5 p. 779-785; DOI 10.1134/S0026893318050035 Full Text

K.S. Antonets1,2, O.P. Onishchuk1, O.N. Kurchak1, K.V. Volkov2, A.N. Lykholay2, E.A. Andreeva2, E.E. Andronov1,2, A.G. Pinaev1, N.A. Provorov1, A.A. Nizhnikov1,2*

Proteomic Profile of the Bacterium Sinorhizobium meliloti Depends on Its Life Form and Host Plant Species

1All-Russia Research Institute of Agricultural Microbiology, St. Petersburg, Pushkin, 196608 Russia
2St. Petersburg State University, St. Petersburg, 199034 Russia

Received - 2018-02-01; Accepted - 2018-03-13

The importance of root nodule bacteria in biotechnology is determined by their distinctive feature: symbiotic nitrogen fixation resulting in the production of organic nitrogen-containing compounds. While interacting with host legume plants, the cells of these bacteria undergo global changes at all levels of expression of genetic information leading to the formation in root nodules of so-called bacteroids functioning as nitrogen fixation factories. The molecular mechanisms underlying plant-microbial symbiosis are actively investigated, and one of the most interesting and poorly studied aspects of this problem is the species-specificity of interaction between root nodule bacteria and host plants. In this work we have performed the proteomic analysis of the Sinorhizobium meliloti bacteroids isolated from two legume species: alfalfa (Medicago sativa L.) and yellow sweet clover (Melilotus officinalis L.). It has been shown that the S. meliloti bacteroids produce a lot of proteins (many of them associated with symbiosis) in a host-specific manner, i.e., only in certain host plant species. It has been demonstrated for the first time that the levels of expression in bacteroids of the genes encoding the ExoZ and MscL proteins responsible for the synthesis of surface lipopolysaccha-rides and formation of a large conductance mechanosensitive channel, respectively, depend on a host plant species that confirms the results of proteomic analysis. Overall, our data show that the regulation of bacteroid development by the host plant has species-specific features.

root nodule bacteria, Sinorhizobium meliloti, Medicago, Melilotus, bacteroid, symbiotic nitrogen fixation, mass spectrometry, chromatography, MALDI, HPLC, MscL, ExoZ