Vol 52(2018) N 4 p. 621-628; DOI 10.1134/S0026893318040088
X. Huang, N. Duan, H. Xu, T.N. Xie, Y.-R. Xue , C.-H. Liu*
CTAB-PEG DNA Extraction from Fungi with High Contents of PolysaccharidesState Key of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210023 China
Received - 2017-03-15; Accepted - 2017-05-19
Polysaccharides influence concentration and purity of extracted DNA. Here we present rapid and efficient protocol for DNA extraction from samples rich in polysaccharides. The technique has been developed using cultures of Schizophyllum commune and involves a modification of known Cetyltrimethyl Ammonium Bromide (CTAB) protocol. To remove polysaccharides, Polyethylene Glycol (PEG) 8000 was added during DNA precipitation. Genomic DNA obtained with the CTAB-PEG method had high integrity, with average fragment size >30 kb, the concentration higher than 100 ng/μL, and the yield more than 30 μg/g. Presented technique is suitable for DNA extraction from fungi, bacteria, archaea or even mollusks with high polysaccharide content.
Fungal genome, CTAB, PEG, polysaccharides, Schizophyllum commune