JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 52(2018) N 4 p. 583-589; DOI 10.1134/S0026893318040143 Full Text

S.V. Nikulin1,2, N.A. Mnafki (Krainova)1, S.A. Shilin1, I.N. Gazizov1, D.V. Maltseva1*

Ribosome Inactivation and the Integrity of the Intestinal Epithelial Barrier

1Scientific Research Center Bioclinicum, Moscow, 115088 Russia
2Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow oblast, 141701 Russia

*d.maltseva@bioclinicum.com
Received - 2017-11-20; Accepted - 2017-12-12

The mistletoe lectin viscumin (MLI) is a ribosome-inactivating protein from Viscum album widely used in cancer therapy. Its antitumor properties are due to its immunomodulating action, previously demonstrated in experiments involving intravenous, subcutaneous, and oral administration of viscumin. To investigate whether viscumin has a cytotoxic effect on the intestinal epithelium, its safety was assessed using (i) impedance spectroscopy to measure the integrity of the colorectal adenocarcinoma Caco-2 cell monolayer after exposure to viscumin and (ii) a novel technique of determining the portion of viscumin-inactivated ribosomes. It was shown that inactivation of at least 20% of the ribosomes within 6 h did not lead to disruption of the Caco-2 cell monolayer or alter the physicochemical parameters of enterocyte membranes.

MLI, viscumin, ricin, ribosome-inactivating protein, impedance spectroscopy, TEER, Caco-2



JMB-FOOTER RAS-JOURNALS