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Vol 48(2014) N 5 p. 734-740; DOI 10.1134/S002689331405015X Full Text

V.M. Tishchenko*

Relations between Macro- and Microstability of CН2 Domains of Human IgG2 and Their Biological Activity: 2. Calculation of Thermodynamic Functions That Characterize the Domain Stability

Institute of Theoretical and Experimental Biophysics Russian Academy of Science, Pushchino, Moscow Region, 142290 Russia

*tischen@vega.protres.ru
Received - 2014-03-14; Accepted - 2014-04-22

Thermodynamic parameters of stabilization of the native structure of CН2 domains in human myeloma second subclass LOM and SIN immunoglobulins and their hFc fragments, which we obtained for the first time, were determined using the method of scanning microcalorimetry. The decrease in thermal stability and energy of stabilization of the native structure of the domains in intact proteins in the physiological range of temperatures (20-37°C) correlated mainly with the entropy factor.

immunoglobulin IgG2, Fc fragment, CН2 domain, thermal stability, enthalpy and entropy of denaturation, free energy of native structure, stabilization



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