Vol 45(2011) N 6 p. 983-992;
GHL1-GHL15: New Families of the Hypothetical Glycoside Hydrolases1State Research Center GosNIIGenetika, Moscow, 117545 Russia
2Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, 117312 Russia
Received - 2011-03-15; Accepted - 2011-05-04
The domains of 15 recently discovered families of the hypothetical glycoside hydrolases GHL1- GHL15 were used for iterative screening of the protein database. The evolutionary relationships between these families were revealed, as well as their relationship with the previously known families of protein domains: GH5, GH13, GH13_33, GH17, GH18, GH20, GH27, GH29, GH31, GH35, GH36A, GH36B, GH36C, GH36D, GH36E, GH36F, GH36G, GH36H, GH36J, GH36K, GH39, GH42, GH53, GH66, GH97, GH101, GH107, GH112, GH114, COG1082, COG1306, COG1649, COG2342, DUF3111, and PF00962. The unclassified homologues were grouped in 35 new families of the hypothetical glycoside hydrolases: GHL16-GHL50. The position of the families GHL1-GHL15 in the hierarchical classification of glycoside hydrolases and their homologues is discussed. Several new superfamilies of protein domains are proposed.
glycoside hydrolase, GHL families, new protein families, protein superfamily, PSI-BLAST, PSI Protein Classifier, CAZy, TIM-barrel fold, hierarchical protein classification, protein evolution, search for homologues, gene annotation