Vol 45(2011) N 6 p. 950-958;
P.V. Spirin1, N.A. Nikitenko1, T.D. Lebedev1, P.M. Rubtsov1, C. Stocking2, V.S. Prasolov1*
Modulation of Activated Oncogene c-kit Expression with RNA-Interference1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia
2Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg, 20251, Germany
Received - 2011-03-30; Accepted - 2011-06-22
Overexpression of oncogene c-kit is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene c-kit was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene c-kit. Further, small hairpin RNAs (shRNAs) targeting c-kit mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in c-kit expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML.
RNA-interference, small interfering RNA (siRNA), small hairpin RNA (shRNA), lentiviral vectors, regulation of gene expression, posttranscriptional regulation of expression, acute myeloid leukemia (AML)