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Vol 51(2017) N 4 p. 614-620; DOI 10.1134/S0026893317040070 Full Text

M.G. Cao1, J. Xu1, Q.F. Yang1, Z. P. Guo1, K.B. Zhang1, X.-B. Li2, S.Q. Wu1, W. Zhou1*

High-Content siRNA Screen of the Kinome Identifies Kinases Involved in Git2-Induced Mesenchymal-Epithelial Transition

1College of Medicine and Health, Lishui University, 323000 China
2College of Engineering and Design, Lishui University, 323000 China

*zhouwu@lsu.edu.cn
Received - 2016-04-27; Accepted - 2016-11-02

Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear. Here, we created a cell based high-content siRNA screen using the breast cancer cell line 4TO7 to search for kinases that were involved in Git2-induced MET. Our results revealed that 58 kinases including transferase, phosphorylation regulators, ATP/nucleotide partners potentially participate in Git2-induced MET. Our preliminary data is expected to facilitate elucidation of the mechanism on how MET is initiated during cancer metastasis.

siRNA screen, kinome, mesenchymal-epithelial transition, Git2



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