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Vol 51(2017) N 4 p. 592-595; DOI 10.1134/S0026893317040173 Full Text

M.V. Tikhonov*, O.G. Maksimenko, P.G. Georgiev, I.V. Korobko

Optimal Artificial Mini-Introns for Transgenic Expression in the Cells of Mice and Hamsters

Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia

*me@mtih.me
Received - 2016-07-24; Accepted - 2016-10-24

Introns can frequently enhance transgene expression, and sometimes they are absolutely substantial. Based on an analysis of murine genes, in which mRNA does not have alternative splicing, a universal design of the efficiently spliced artificial introns of small sizes has been proposed. These introns are shown to be efficiently spliced in CHO cells from hamster ovaries. The proposed strategy can be used to include introns in cDNA, which would elevate the production of recombinant proteins in cell culture, as well as in transgenic animals.

artificial intron, cDNA, expression



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