Vol 51(2017) N 4 p. 514-525; DOI 10.1134/S0026893317040033
A.V. Bannikov1*, A.V. Lavrov1,2
CRISPR/CAS9, the King of Genome Editing Tools1Medical Genetic Research Center, Moscow, 115478 Russia
2Pirogov Russian National Research Medical University, Moscow, 117997 Russia
Received - 2016-06-15; Accepted - 2016-10-27
The discovery of CRISPR/Cas9 brought a hope for having an efficient, reliable, and readily available tool for genome editing. CRISPR/Cas9 is certainly easy to use, while its efficiency and reliability remain the focus of studies. The review describes the general principles of the organization and function of Cas nucleases and a number of important issues to be considered while planning genome editing experiments with CRISPR/Cas9. The issues include evaluation of the efficiency and specificity for Cas9, sgRNA selection, Cas9 variants designed artificially, and use of homologous recombination and nonhomologous end joining in DNA editing.
CRISPR/Cas9, genome editing, sgRNA