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Vol 44(2010) N 5 p. 776-786; P.V. Spirin1, D. Baskaran1, N.N. Orlova1, A.V. Rulina1, N.A. Nikitenko1, E.L. Chernolovskaya3, M.A. Zenkova3, V.V. Vlasov3, P.M. Rubtsov1, P.M. Chumakov1, C. Stocking2, V.S. Prasolov1* Downregulation of Activated Leukemic Oncogenes AML1-ETO and RUNX1(K83N) Expression with RNA-Interferenc 1Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Moscow, 119991, Russia2Heinrich-Pette-Institute for Experimental Virology and Immunology, Hamburg, 20251, Germany 3Institute of Chemical biology and Fundamental Medicine of Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia *prasolov@eimb.ru Received - 2010-01-25; Accepted - 2010-04-08 In the present study, we have applied the siRNA approach to reduce the expression of AML1-ETO and RUNX1(K83N) oncogenes, which are frequently found in leukemic cells. We have designed small hairpin RNAs (shRNA) for targeting AML1-ETO oncogene and a region close to the 5'-untranslated region of mRNA for the mutant RUNX1(K83N) oncogene and expressed the shRNAs in lentiviral vectors. We report a stable reduction in expression of oncogenes following the introduction of shRNAs into cells. regulation of gene expression, posttranscriptional regulation of expression, RNA-interference, acute myeloid leukemia (AML), siRNA, shRNA, lentiviral vectors |
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