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Vol 47(2013) N 4 p. 524-529; A.A. Guskova1*, M.Y. Skoblov1,2, A.V. Lavrov1, D.A. Zubtsov3, V.L. Andronova4, D.V. Goldstein1, G.A. Galegov4, Y.S. Skoblov5 Molecular Genetic Analysis of DNA Polymerase and Thymidine Kinase Genes of a HSV-1 Population Using an MPS Technology 1Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moscow, 115478 Russia2Moscow State University of Medicine and Dentistry, Moscow, 127473 Russia 3Moscow Institute of Physics and Technology, Dolgoprudnyi, 141700 Russia 4Ivanovskii Institute of Virology, Russian Academy of Medical Sciences, Moscow, 123098 Russia 5Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia *gousskova@gmail.com Received - 2012-11-20; Accepted - 2013-02-08 Real-time PCR was used to determine the ratio of viral and host DNA in lysates of Vero cells infected with HSV-1 strain L2. The number of virus copies reached a maximum after 24 h of incubation. Total isolated DNA was sequenced using the massively parallel sequencing technique on an Ion Torrent apparatus. Nucleotide sequences of thymidine kinase (UL23) and DNA polymerase (UL30) genes of a HSV-1 L2 population were determined; their primary structures were compared to those of other standard HSV-1 strains, KOS and 17. The detected differences between the UL23 and UL30 sequences of L2 and reference strains KOS and 17 were unimportant because these substitutions did not affect the conserved gene regions. DNA polymerase, thymidine kinase, herpes simplex virus type 1, MPS technology |
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