JMB-HEADER RAS-JOURNALS EIMB Pleiades Publishing

RUS

             

ENG

YearIMPACT-FACTOR
2022  1,200
2021  1,540
2020  1,374
2019  1,023
2018  0,932
2017  0,977
2016  0,799
2015  0,662
2014  0,740
2013  0,739
2012  0,637
2011  0,658
2010  0,654
2009  0,570
2008  0,849
2007  0,805
2006  0,330
2005  0,435
2004  0,623
2003  0,567
2002  0,641
2001  0,490
2000  0,477
1999  0,762
1998  0,785
1997  0,507
1996  0,518
1995  0,502
Vol 47(2013) N 3 p. 352-357;
M.S. Nazarenko1,2*, A.V. Markov1, I.N. Lebedev1,2, A.A. Sleptsov1, A.V. Frolov3, O.L. Barbarash3, L.S. Barbarash3, V.P. Puzyrev1,2

DNA Methylation Profiling of the Vascular Tissues in the Setting of Atherosclerosis

1Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, 634050 Russia
2Siberian State Medical University, Ministry of Healthcare of the Russian Federation, Tomsk, 634050 Russia
3Research Institute for Complex Studying of Cardiovascular Diseases, Russian Academy of Medical Sciences, Kemerovo, 650002 Russia

*maria.nazarenko@medgenetics.ru
Received - 2012-09-26; Accepted - 2012-10-11

Currently, the question of epigenetic mechanisms of gene regulation in the context of cardiovascular diseases is of considerable interest. Here, DNA methylation profiles of vascular tissues of atherosclerotic patients have been analyzed for the first time using the Infinium Human Methylation27 BeadChip microarray (Illumina, United States). As the result, within 286 genes, 314 CpG sites that varied significantly in the level of DNA methylation between the tissue samples of carotid (in the area of atherosclerotic plaques and nearby macroscopically intact tissues of the vascular wall) and mammary arteries, as well as saphenous veins have been identified. The most pronounced differences in the methylation level was registered for CpG sites of homeobox genes HOXA2 and HOXD4, as well as the imprinted MEST gene. In particular, these genes were found to be hypomethylated in the carotid atherosclerotic plaques compared to their methylation patterns in intact tissues of internal mammary arteries and saphenous veins.

DNA methylation, atherosclerosis, Infinium Human Methylation27 BeadChip



JMB-FOOTER RAS-JOURNALS