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Vol 47(2013) N 1 p. 97-104;
N.N. Ryzhova1*, M.A. Filiushin1, A.M. Artemeva2, M.V. Berdnikova3, V.V. Taranov3, A.V. Babakov3, E.Z. Kochieva1

Identification and Nucleotide Polymorphisms in Brassica rapa Genes Coding Cold-Shock Domain Proteins

1Center «Bioengineering», Russian Academy of Sciences, Moscow, 117312 Russia
2Vavilov All-Russia Institute of the Plant Industry, Russian Academy of Agricultural Sciences, St. Petersburg, 190000 Russia
3Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow, 127550 Russia

*rynatalia@yandex.ru
Received - 2012-04-17; Accepted - 2012-06-13

Full-length BrCSDP2 and BrCSDP4 cold-shock gene sequences of Brassica rapa have been obtained. The isolated genes were shown to belong to a group AtCSP2/AtCSP4 of Arabidopsis thaliana and TsCSDP2/TsCSDP4 of Thellungiella salsuginea genes that encode proteins with a cold-shock domain and two zinc finger motifs. The structure and the allelic variants of these genes have been described and characterized. The identified allelic polymorphism was shown to result both from point substitutions and small indels. Coefficients of total genetic similarity ranged from 1.0 to 0.53. For BrCSDP2 and AtCSDP2, the genetic similarity coefficient was 0.89 and, for BrCSDP4 and AtCSDP4, it was 0.85. The in silico translation of gene sequences has revealed amino acid substitutions in the protein sequence, but no significant correlation between the detected polymorphism and signs of resistance to cold stress were found.

cold shock genes, structure and allelic polymorphism, turnips, Brassicaceae, nucleotide and amino acid substitutions, indels, evolution and phylogeny



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